Recent progress in cancer cell membrane-based nanoparticles for biomedical applications

• Qixiong Lin,
• Yueyou Peng,
• Yanyan Wen,
• Xiaoqiong Li,
• Donglian Du,
• Weibin Dai,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2023, 14, 262–279, doi:10.3762/bjnano.14.24

• cancer cell membrane protein components (b), which were similar to the cell lysate (c). (B) The box plot shows that the dimensional stability of cancer cell membrane biomimetic nanoparticles in water and PBS is significantly higher than that of bare nanoparticles. Adapted from [31]. (© 2019 Liu X et al

pH-driven enhancement of anti-tubercular drug loading on iron oxide nanoparticles for drug delivery in macrophages

• Karishma Berta Cotta,
• Sarika Mehra and
• Rajdip Bandyopadhyaya

Beilstein J. Nanotechnol. 2021, 12, 1127–1139, doi:10.3762/bjnano.12.84

• cells, i.e., the macrophages, were then treated with 32 µg/mL of NOR, in either free or nanoparticle-coated form, for 48 h. The extracellular drug and nanoparticles were washed off with ice cold PBS and cells lysed overnight, with 0.1 N glycine–HCl, at pH 3.5. A fixed volume of each cell lysate was

Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• buffer (1× RIPA buffer, PMSF, 1% protease and protease inhibitors cocktail, Cell Signaling Technology, USA) and quantified using Lowry’s method. An aliquot of the cell lysate containing 50 µg protein was loaded in 12% sodium dodecyl sulfate–polyacrylamide gel. The membrane was blocked for 1 h with

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

• Arianna Gennari,
• Julio M. Rios de la Rosa,
• Erwin Hohn,
• Maria Pelliccia,
• Enrique Lallana,
• Roberto Donno,
• Annalisa Tirella and
• Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

• after cell lysate centrifugation (4,500 rpm, 2 min) using the ONE Glo luciferase assay following manufacturer’s instructions. The relative luminescence units (RLU) were measured using a Synergy2 Biotek plate reader (Gen5 data acquisition software), and normalized against the total protein content (BCA

Surface-enhanced Raman spectroscopy of cell lysates mixed with silver nanoparticles for tumor classification

• Mohamed Hassoun,
• Iwan W.Schie,
• Tatiana Tolstik,
• Sarmiza E. Stanca,
• Christoph Krafft and
• Juergen Popp

Beilstein J. Nanotechnol. 2017, 8, 1183–1190, doi:10.3762/bjnano.8.120

• silver nanoparticles. Keywords: cell lysate; silver nanoparticles; surface-enhanced Raman spectroscopy (SERS); tumor-cell differentiation; Introduction Cytopathology is the histopathologic inspection of cells. Dyes, such as hematoxylin for cell nuclei or eosin for cytoplasm, are commonly used to stain

• . The sonication was applied in 3 cycles of 15 s each and 5 s break in between with a power set to 20%. The cell lysate was then transferred to a new tube and stored until further processing in a freezer. Raman spectroscopy and SERS measurements SERS measurements were performed on a commercial Raman

• white light source. The laser power was fixed at 50 mW with an acquisition time of 5 s. Each batch was lysed and divided into eight to ten samples. 100 µL of the silver nanoparticles were mixed with 100 µL KCl as aggregating agent, and then 100 µL of cell lysate were added to the mixture with a final

Novel ZnO:Ag nanocomposites induce significant oxidative stress in human fibroblast malignant melanoma (Ht144) cells

• Syeda Arooj,
• Samina Nazir,
• Akhtar Nadhman,
• Nafees Ahmad,
• Bakhtiar Muhammad,
• Ishaq Ahmad,
• Kehkashan Mazhar and
• Rashda Abbasi

Beilstein J. Nanotechnol. 2015, 6, 570–582, doi:10.3762/bjnano.6.59

• maintained on ice until it was further assayed. To the cell lysate (0.1 mL) an equal amount of 10% SDS was added and samples were incubated at room temperature for 5 min. Then, 0.25 mL of TBA (5.2 mg/mL) was added to the mixture and incubated at 95 °C for 45 min. After cooling to room temperature, absorbance

Functionalization of α-synuclein fibrils

• Simona Povilonienė,
• Vida Časaitė,
• Virginijus Bukauskas,
• Arūnas Šetkus,
• Juozas Staniulis and
• Rolandas Meškys

Beilstein J. Nanotechnol. 2015, 6, 124–133, doi:10.3762/bjnano.6.12

• ), containing 0.1 mM EDTA, 0.2 mM PMSF, and 500 mM NaCl. The cell lysate was heated at 100 °C for 10 min and subsequently centrifuged at 13200g for 20 min. The supernatant was dialyzed against 50 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 1 mM EDTA, pH 8.0, and loaded into a HiTrap ANX column (GE Healthcare